Coding

Part:BBa_K3286201:Design

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-19)


RpfG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1091
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A silent mutation was needed for amino acid 176. Here the GAG needed to be mutated in a GAA (both transcribing for Glutamic acid). This was needed to make this part TypeIIS compatible.


Source

The original part came from the iGEM repository.

References